OrangerV1, Main, Exploration, bibRecord, 002619

Vacuolar citrate/H+ symporter of citrus juice cells

Identifieur interne : 002619 ( Main/Exploration ); précédent : 002618; suivant : 002620

Vacuolar citrate/H+ symporter of citrus juice cells

Auteurs : Takehiko Shimada [États-Unis] ; Ryohei Nakano [États-Unis] ; Vladimir Shulaev [États-Unis] ; Avi Sadka [Israël] ; Eduardo Blumwald [États-Unis]

Source :

Planta [ 0032-0935 ] ; 2006.

RBID : Pascal:06-0346660

Descripteurs français

Pascal (Inist)
- Citrate, DNA complémentaire, Vacuole, Homéostasie, Citrus sinensis, Citrique acide.

English descriptors

KwdEn :
- Amino Acid Sequence, Beverages, Blotting, Northern, Blotting, Western, Citrate, Citrates (metabolism), Citric acid, Citrus (chemistry), Citrus (cytology), Citrus sinensis, Complementary DNA, Homeostasis, Hydrogen-Ion Concentration, Molecular Sequence Data, Protein Transport, RNA, Messenger (genetics), RNA, Messenger (metabolism), Symporters (chemistry), Symporters (genetics), Symporters (metabolism), Vacuole, Vacuoles (metabolism).
MESH :
- chemical , chemistry : Symporters.
- chemical , genetics : RNA, Messenger, Symporters.
- chemical , metabolism : Citrates, RNA, Messenger, Symporters.
- chemistry : Citrus.
- cytology : Citrus.
- metabolism : Vacuoles.
- Amino Acid Sequence, Beverages, Blotting, Northern, Blotting, Western, Homeostasis, Hydrogen-Ion Concentration, Molecular Sequence Data, Protein Transport.

Abstract

We have isolated a cDNA, designated Citrus sinensis citrate transporter 1 CsCit1 encoding a novel vacuolar citrate/symporter. Immunoblots using antibodies raised against CsCit1 showed that the protein is localized to the juice sac cell vacuoles. The highest expression of CsCit1 and the amount of protein in the juice sac cell vacuoles coincided with the developmental stage at which the vacuolar citrate content began declining with the concomitant increase in vacuolar pH. Vacuoles from Sacharomyces cereviseae expressing CsCit1 displayed a citrate-dependent H⁺ efflux, and our results clearly demonstrate that CsCit1 is able to mediate the electroneutral co-transport of H⁺ and citrate ions, since the citrate-dependent H⁺ fluxes are not affected by changing the electrical potential difference across the tonoplast. The roles of CsCit1 in mediating citrate efflux from the vacuole and on citric acid homoestasis in Citrus juice sac cells are discussed.

Affiliations:

Le document en format XML

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<author><name sortKey="Shimada, Takehiko" sort="Shimada, Takehiko" uniqKey="Shimada T" first="Takehiko" last="Shimada">Takehiko Shimada</name>
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 symporter of citrus juice cells</title>
<author><name sortKey="Shimada, Takehiko" sort="Shimada, Takehiko" uniqKey="Shimada T" first="Takehiko" last="Shimada">Takehiko Shimada</name>
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<term>Citrate</term>
<term>Citrates (metabolism)</term>
<term>Citric acid</term>
<term>Citrus (chemistry)</term>
<term>Citrus (cytology)</term>
<term>Citrus sinensis</term>
<term>Complementary DNA</term>
<term>Homeostasis</term>
<term>Hydrogen-Ion Concentration</term>
<term>Molecular Sequence Data</term>
<term>Protein Transport</term>
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<term>RNA, Messenger (metabolism)</term>
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<term>Symporters (genetics)</term>
<term>Symporters (metabolism)</term>
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<term>Vacuoles (metabolism)</term>
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<term>Beverages</term>
<term>Blotting, Northern</term>
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">We have isolated a cDNA, designated Citrus sinensis citrate transporter 1 CsCit1 encoding a novel vacuolar citrate/symporter. Immunoblots using antibodies raised against CsCit1 showed that the protein is localized to the juice sac cell vacuoles. The highest expression of CsCit1 and the amount of protein in the juice sac cell vacuoles coincided with the developmental stage at which the vacuolar citrate content began declining with the concomitant increase in vacuolar pH. Vacuoles from Sacharomyces cereviseae expressing CsCit1 displayed a citrate-dependent H<sup>+</sup>
 efflux, and our results clearly demonstrate that CsCit1 is able to mediate the electroneutral co-transport of H<sup>+</sup>
 and citrate ions, since the citrate-dependent H<sup>+</sup>
 fluxes are not affected by changing the electrical potential difference across the tonoplast. The roles of CsCit1 in mediating citrate efflux from the vacuole and on citric acid homoestasis in Citrus juice sac cells are discussed.</div>
</front>
</TEI>
<affiliations><list><country><li>Israël</li>
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<name sortKey="Blumwald, Eduardo" sort="Blumwald, Eduardo" uniqKey="Blumwald E" first="Eduardo" last="Blumwald">Eduardo Blumwald</name>
<name sortKey="Nakano, Ryohei" sort="Nakano, Ryohei" uniqKey="Nakano R" first="Ryohei" last="Nakano">Ryohei Nakano</name>
<name sortKey="Shulaev, Vladimir" sort="Shulaev, Vladimir" uniqKey="Shulaev V" first="Vladimir" last="Shulaev">Vladimir Shulaev</name>
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</record>

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Vacuolar citrate/H+ symporter of citrus juice cells

Vacuolar citrate/H+ symporter of citrus juice cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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